Laccase TEA experimental data

Author(s): Elmin Rahic, Nicholas Cassady, Zhiyou Wen, Kurt Rosentrater

Abstract:

Laccase fermentation was conducted by cultivating white-rot fungi (Pleurotus ostreatus) on prairie biomass (growth phase), followed by the induction of laccase using bio-oil aqueous phase (induction phase). This fermentation process was modeled for techno-economic analysis based on experimental data. The experimental data consisted of two parts: 1) optimizing nitrogen concentration for the growth phase, and 2) modeling laccase production and induction phase time using response surface methodology by varying substrate bed depth, substrate to inoculum ratio, and growth phase length.

Funder: USDA NIFA

Award Title:

Consortium for Cultivating Human And Naturally reGenerative Enterprises: Grass2Gas

Award Number: 2020-68012-31824

Keywords:

Pleurotus ostreatus, bio-oil aqueous phase, biochar, corn steep, laccase, prairie biomass

Project (Abbreviated name):

G2G_Rosentrater_laccase_TEA_experimental_data

Team: Grass2Gas

Families:

Pleurotaceae

Species:

Pleurotus ostreatus

Full Metadata:

G2G_Rosentrater_laccase_TEA_experimental_data metadata.pdf (252.13 KB)

Data Mirror:

Data Table Column Names and Descriptions

Table name	Column name	Data type	Units	Column description
data.dataset_fungal_growth_by_corn_steep_concentration	average mass g	float	gram	Mean fungal biomass over three trials.
data.dataset_fungal_growth_by_corn_steep_concentration	corn steep concentration g per l	float	gramPerLiter	Mass of corn steep solids mixed with media, expressed in grams per liter.
data.dataset_fungal_growth_by_corn_steep_concentration	dataset fungal growth by corn steep concentration pkey	integer	dimensionless	A unique primary key for this table.
data.dataset_fungal_growth_by_corn_steep_concentration	fungal growth mass g	float	gram	Mycelial biomass was harvested by scraping and quantifying the biomass gravimetrically. Expressed in grams.
data.dataset_fungal_growth_by_corn_steep_concentration	standard deviation g	float	gram	Standard deviation of the mean fungal biomass over three trials.
data.dataset_fungal_growth_by_nitrogen_source	dataset fungal growth by nitrogen source pkey	integer	dimensionless	A unique primary key for this table.
data.dataset_fungal_growth_by_nitrogen_source	fungal biomass g	float	gram	Mycelial biomass was harvested by scraping and quantifying the biomass gravimetrically. Expressed in grams.
data.dataset_fungal_growth_by_nitrogen_source	nutrient	string	NA	The nitrogen source tested.
data.dataset_model_result_table	coefficient	string	NA	The variable used to express this parameter in the central composite design model.
data.dataset_model_result_table	dataset model result table pkey	integer	dimensionless	A unique primary key for this table.
data.dataset_model_result_table	estimate	float	dimensionless	The parameter estimate produced by the model.
data.dataset_model_result_table	f value	float	dimensionless	The F statistic reported by the model.
data.dataset_model_result_table	p value	string	NA	The p value reported by the model.
data.dataset_model_result_table	variable	string	NA	The experimental condition, its transformation (if any), and interaction with other variables.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	absorbance after 0 seconds	float	NA	Spectrophotometer absorbance at the indicated reaction time. Absorbance through time was used to calculate laccase yield using the equation provided in the Analysis subsection of Methods.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	absorbance after 120 seconds	float	NA	Spectrophotometer absorbance at the indicated reaction time. Absorbance through time was used to calculate laccase yield using the equation provided in the Analysis subsection of Methods.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	absorbance after 150 seconds	float	NA	Spectrophotometer absorbance at the indicated reaction time. Absorbance through time was used to calculate laccase yield using the equation provided in the Analysis subsection of Methods.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	absorbance after 180 seconds	float	NA	Spectrophotometer absorbance at the indicated reaction time. Absorbance through time was used to calculate laccase yield using the equation provided in the Analysis subsection of Methods.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	absorbance after 30 seconds	float	NA	Spectrophotometer absorbance at the indicated reaction time. Absorbance through time was used to calculate laccase yield using the equation provided in the Analysis subsection of Methods.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	absorbance after 60 seconds	float	NA	Spectrophotometer absorbance at the indicated reaction time. Absorbance through time was used to calculate laccase yield using the equation provided in the Analysis subsection of Methods.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	absorbance after 90 seconds	float	NA	Spectrophotometer absorbance at the indicated reaction time. Absorbance through time was used to calculate laccase yield using the equation provided in the Analysis subsection of Methods.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	culture time hours	float	hour	Time since induction with bio-oil aqueous phase, expressed in hours.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	dilution factor	integer	number	The number of times the sample was diluted before testing to keep absorbances within the range that could be read by the spectrophotometer.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	id number	integer	dimensionless	The assigned experimental ID. Each combination of substrate bed depth, substrate to inoculum ratio, and pre-induction growth time was assigned a unique ID.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	laccase activity units per g	float	NA	The calculated laccase activity expressed in units per gram prairie biomass. Activity was calculated using the formula presented in the methods section.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	laccase activity units per g mean	float	NA	The calculated laccase activity averaged over two replicates, expressed in units per gram prairie biomass. Activity was calculated using the formula presented in the methods section.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	laccase activity units per ml	float	NA	The calculated laccase activity expressed in units per milliliter SUBSTANCE. Activity was calculated using the formula presented in the methods section.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	laccase by bed depth s i ratio and time raw pkey	integer	dimensionless	A unique primary key for this table.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	molar extinction coefficient	float	NA	A measurement of how strongly a substance absorbs light, expressed as M-1cm-1. Used to estimate the rate of color change (i.e. absorbance) to estimate laccase activity.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	slope absorbance per minute	float	NA	The slope of the best fit line drawn through absorbance by reaction time. I.e., mean change in absorbance through time.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	slope r squared	float	NA	The proportion of the variation in the absorbances that was predictable from the reaction time along the best fit line. In other words, how well the slope fit the data.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	substrate bed depth cm	float	centimeter	The depth of the prairie biomass during the growth phase expressed in centimeters.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	substrate inoculum ratio g per g	float	gramPerGram	The ratio of prairie biomass substrate to P. ostreatus spawn used, expressed in grams per gram.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	time days	float	NA	The duration of the pre-induction culture phase expressed in days.
data.laccase_by_bed_depth_s_i_ratio_and_time_raw	total volume to sample volume ratio	float	dimensionless	Ratio of total volume (Vt) to sample volume (Vs) during the laccase activity assay. Used to convert the volume of laccase input for the assay (20-40 uL) to 1 mL.